Coding

Part:BBa_K4728006:Design

Designed by: Michelle Li   Group: iGEM23_Queens-Canada   (2023-10-08)


TphA1 (Reductase)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 433
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 436
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 433
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 433
    Illegal AgeI site found at 366
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

TphA1 comes from the species Comammonas sp. Strain E6.

References

Sasoh, M., Masai, E., Ishibashi, S., Hara, H., Kamimura, N., Miyauchi, K., & Fukuda, M. (2006). Characterization of the terephthalate degradation genes of Comamonas sp. strain E6. Applied and environmental microbiology, 72(3), 1825–1832. https://doi.org/10.1128/AEM.72.3.1825-1832.2006